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Division of Reproductive Engineering
We seek to unravel mechanisms of reproductive biology and make
innovations in reproductive technology in mammals.
We solved critical problems in mouse reproductive technology which is widely used in transgenic facilities and mouse repositories. Our technology is available from our online manual. In addition, we provide service of mouse repository (CARD Mouse Bank) which collaborates with our international partners.
News
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Feburary 7, 2023:
- September 18, 2020:
Our review paper about "Cryopreservation of Mouse Resources" is published in Laboratory Animal Research. - August 28, 2020:
Registration of the virtual meeting of International Society for Transgenic Tchnologies is opened. - June 1, 2020:
Our paper was published about New microfluidics technology for sperm sorting (Nakao, Scientific Reports, 2020).
Our paper was published about "Synchronization of the ovulation and copulation timings increased the number of in vivo fertilized oocytes in superovulated female mice" in PLoS One.
Feburary 1, 2023:
Our paper was published about "Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm" in Biology of Reproduction.
Feburary 1, 2023:
Our paper was published about "Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm" in Biology of Reproduction.
January 19, 2023:
Our paper was published about "Observation of the in vitro fertilization process in living oocytes using frozen-thawed sperm in rats" in Theriogenology.
October 13, 2022:
Our paper about "Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat" was published in Lab Animal.
ShareIt Link: https://rdcu.be/cXgma
↓Animation Abstract↓ June 14, 2022:
We won an award from the Minister of Education, Culture, Sports, Science, and Technology (MEXT) in the Program of Innovation Net Award. I am grateful to all of our staff members, collaborators, and supporters.
November 22, 2021:
Our paper was published about "Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days" in Scientific Reports.
September 6, 2021:
Our collaboration paper about "Asian Mouse Mutagenesis Resource Association (AMMRA): mouse genetics and laboratory animal resources in the Asia Pacific" is now published in Mammalian Genome. Thank you to all our partners and stakeholders for supporting the Asia Mouse Mutagenesis and Resource Association (#AMMRA).
August 6, 2021:We have a new lab member. Welcome, Kahar. Let's explore the new frontier of Reproductive Science.
June 3, 2021:
CARD Rat Bank officially started a service of "Sperm cryopreservation and In vitro fertilization" to preserve and supply rat resources.
Feburary 18, 2021:
Our collaboration paper is published about "Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation" in Nature Metabolism.
Nombember 14, 2020:
Our lab's movie is uploaded on "YouTube Channel of Graduate School of Medical Sciences".
Nombember 13, 2020:
We opened "【Takeo Lab】Div. of Reproductive Engineering YouTube Channel".
Our Mission
- -Discover novel compounds and materials to regulate
- reproductive functions
- -Comprehend unrevealed rules in Reproductive Biology and
- Cryobiology
- -Provide solutions in Reproductive Technology
- -Develop a robust system and network of bioresources (Mice and Rats)
- -Share the latest techniques and knowledge of Reproductive Technology
- -Grow young scientists and technicians in Reproductive Technology
Research
- -L-glutamine protected mouse sperm during cryopreservation (Lab Animal, 2010).
- -Methyl-beta-cyclodextrin enhanced fertilization rate of frozen-thawed sperm (Biol Reprod, 2008).
- -Reduced glutathione enhanced fertilizing ability of oocyte (Biol Reprod, 2011).
- -Coadministratin of inhibin antiserum and gonadotropin induced ultrasuperovulation (PLoS One, 2015).
- -Dimethyl sulfoxide and quercetin prolonged survival period of sperm at 4oC (Biol Reprod, 2017).
- -N-acetyl cysteine prolonged survival period of two-cell embryos at 4oC (Cryobiology, 2016).
- -N-acetyl cysteine restored fertilizing ability of cryopreserved oocyte (PLoS One, 2019).
- -Microfludics cell sorter selected sperm with high fertility (Scientific Reports, 2020).
Access to CARD Reproductive Technology
- We publish all our techniques of in vitro fertilization, sperm and embryo cryopreservation, cold-storage of sperm and embryos, embryo transfer, and so on in Online Manual for CARD Mouse Reproductive Technology. In addition, non-profit organization can be obtained ultrasuperovulation reagent after agreement of MTA.
CARD Mouse Bank
- We provide service of mouse embryo and sperm bank
- (CARD Mouse Bank) since 1998. CARD Mouse Bank is a founding member of International Mouse Strain Resource (IMSR). We developed original searchable database of genetically engineered mice archived in CARD Mouse Bank (CARD R-BASE). Totally, 2,239 strains of genetically engineered mice are available.
- If you want to order the mice, please visit our ordering website.
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Our Team
| Name | |||
| Professor | Toru Takeo | takeo* | Grant and Publication list etc. |
| Visiting Professor | Jorge M Sztein | Grant and Publication list etc. | |
| Assistant Professor | Yoshiko Nakagawa | y-naka* | Grant and Publication list etc. |
| Research Fellow | Satohiro Nakao | naka-sato* | Grant and Publication list etc. |
| Research Fellow | Kaharul Kahar | ||
| IT Specialist | Shuuji Tsuchiyama | shuuji* | Grant and Publication list etc. |
| Breeding Manager | Wataru Sakamoto | watarus* | |
| Secretary | Fumi Takahashi | card-rep* | |
| Import and Export Coordinator | Mari Iwamoto | card* | |
| Breeding Facility Receptionist | Kaori Sakaguchi | cardadmi* | |
| Mouse Bank Technician | Satoshi Nakamura | ||
| Mouse Bank Technician | Kiyoko Yamashita | ||
| Mouse Bank Technician | Maki Sakaguchi | ||
| Mouse Bank Technician | Yuka Deshimaru | ||
| Mouse Bank Technician | Kiharu Uchigoshi | ||
| Mouse Bank Technician | Kodai Uno | ||
| Mouse Bank Technician | Keisuke Kogami | Mouse Bank Technician | Erina Maruoka |
| Graduate Student | Kotono Ito | ||
| Graduate Student | Serina Kuroshima | ||
| R＆D Team Leader Graduate Student |
Katsuma Yamaga | ||
| Graduate Student | Ryo Kubota | ||
| Graduate Student | Ryusei Maeda | ||
| Graduate Student | Ayana Shitashimizu | ||
| Undergraduate Student | Yoshino Wakasugi | ||
| Undergraduate Student | Reiri Koga | ||
| Undergraduate Student | Sakura Nakamitsu | ||
| Undergraduate Student | Keisuke Masuda | PLEASED Program |
